SOFT AGAR ASSAY FOR COLONY FORMATION . Note: All volumes are calculated to cater for four plates per point. Base Agar . 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 °C in a water bath. Agar 2.0 g Distilled water 100 ml Adjust pH to 6.0. Steam the medium to melt agar and add the following ... boiled for 15-20 min in boiling water bath and cooled.

Aug 02, 2016 · (B) In the second compartment, the material is stored in a 150°F (65°C) water bath until needed. Material can be stored for as long as several days. (C) Several minutes before it is to be used for an impression, the reversible hydrocolloid is placed in a 110°F (45°C) water bath. This step is called tempering. May 27, 2014 · Warm the agar bottle in a hot water bath or in the microwave until it becomes liquid. After opening the cap, pass the neck of the agar bottle through a flame to sterilize it. Do not lose the cap! While pouring the agar, open the Petri dish lid as little as possible, hold it at an angle, and make sure the lid is kept directly over the Petri dish.